Screening and Molecular Identification of Novel Pectinolytic Bacteria from Forest Soil

Pectinases are a group of enzymes with broad application, including in plant fiber processing, pectic wastewater treatment, paper pulping, fruit juice extraction, and clarification. With an increasing industrial demand for these enzymes, it is useful to isolate organisms that produce large amounts pectinase possess wide ranges stability factors like temperature pH. In this study, 17 out 29 bacteria (58.62%) from forest soil samples were pectinolytic. However, only four (S-5, S-10, S-14, S-17) showed high pectin hydrolysis zones (ranging 0.2 cm 1.7 cm). These identified based on colony morphology, microscopic characterization, biochemical characteristics, 16S rDNA sequencing. They designated as Streptomyces sp. S-14), Cellulomonas (S-10), Bacillus (S-17). Interestingly, cellulase xylanase activity addition pectinase. The quantitative assay the isolates provided proof they producers can be considered potential candidates uses. crude enzyme extracts applicable oil extraction sesame seeds apples, respectively.